華雅旗下紅榮微再授權(quán)代理（上海）TAKARA CellArtis，Rubicon產(chǎn)品 

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圖片為展示效果，實(shí)際三個(gè)產(chǎn)品都是一樣的規(guī)格。

監(jiān)控人類細(xì)胞移植用途的抗體

(Antibodies for Detecting Human Cells Transplanted into Mice and Rats)

STEM101、STEM121、STEM123是三種小鼠來源的單克隆抗體，分別對人細(xì)胞核標(biāo)記蛋白、人細(xì)胞質(zhì)標(biāo)記蛋白和人GFAP具有特異性，用于人類細(xì)胞向小鼠和大鼠移植實(shí)驗(yàn)中，監(jiān)控細(xì)胞移植、轉(zhuǎn)移、分化。

STEM101®

Mouse Monoclonal Antibody Specific for Human Cell Nucleus Marker

小鼠單克隆抗體抗人細(xì)胞核標(biāo)記

STEM121®

Mouse Monoclonal Antibody Specific for Human Cytoplasmic Marker

小鼠單克隆抗體抗人細(xì)胞質(zhì)標(biāo)記

STEM123®

Mouse Monoclonal Antibody Specific for Human GFAP

小鼠單克隆抗體抗人GFAP

STEM101®  Y40400 50 μg

STEM121®  Y40410 50 μg

STEM123®  Y40420 50 μg

可分開購買

GFAP：膠質(zhì)纖維酸性蛋白（glial fibrillary acidic protein）

是星形膠質(zhì)細(xì)胞活化的標(biāo)志物。主要分布于中樞神經(jīng)系統(tǒng)

產(chǎn)品特點(diǎn)

STEM101與人類細(xì)胞核Ku80蛋白特異性反應(yīng)， 不與小鼠或大鼠腦組織或細(xì)胞提取物中的Ku80交叉反應(yīng)

STEM121與在腦，肝臟，胰腺和CNS細(xì)胞中表達(dá)的人類細(xì)胞質(zhì)蛋白反應(yīng)，該抗體不與來自小鼠，大鼠或食蟹猴的腦組織或細(xì)胞提取物交叉反應(yīng)

STEM123與人類GFAP（一種星形膠質(zhì)細(xì)胞的標(biāo)志物）特異性反應(yīng)，不與來自小鼠或大鼠的腦組織或細(xì)胞提取物交叉反應(yīng)

STEM101 reacts specifically with the nuclear human Ku80 protein; the antibody does not cross-react with mouse or rat Ku80 in brain tissue or cell extracts

STEM121 reacts with a human cytoplasmic protein expressed in brain, liver, pancreas, and CNS cells the antibody does not cross-react with brain tissue or cell extracts from mouse, rat, or cynomologous monkey

STEM123 reacts specifically with human GFAP, a marker of astroglial cells; the antibody does not cross-react with brain tissue or cell extracts from mouse or rat

產(chǎn)品應(yīng)用

通過免疫組織化學(xué)和免疫熒光法定位、定量、表征人源的移植細(xì)胞

Localization, quantification, and/or characterization of engrafted cells of human origin by immunohistochemistry and immunofluorescence

STEM101 STEM121 STEM123已廣泛用于檢測移植到小鼠和大鼠中人類細(xì)胞的植入、遷移和分化?？赏ㄟ^免疫組織化學(xué)來確定移植細(xì)胞的位置和數(shù)量。

產(chǎn)品參數(shù)

Size:50 μg

體積:100 μL

同種型Isotype:lgG1

Form:Unconjugated

特異性：

STEM101 與人類細(xì)胞核蛋白特異反應(yīng)，不與小鼠大鼠腦組織或細(xì)胞提取物交叉反應(yīng)。

STEM121 與人類細(xì)胞質(zhì)蛋白特異反應(yīng)，不與小鼠大鼠獼猴腦組織或細(xì)胞提取物交叉反應(yīng)

STEM123 與人類GFAP蛋白特異反應(yīng)， 不與小鼠大鼠腦組織或細(xì)胞提取物交叉反應(yīng)。

移植監(jiān)控表現(xiàn)

STEM101 detects nuclei of transplanted human neural stem cells in the olfactory bulb of a mouse brain.

STEM101在小鼠腦的嗅球中檢測移植的人類神經(jīng)干細(xì)胞的細(xì)胞核。

STEM121 detects migration and differentiation of transplanted human neural stem cells in the hippocampus of a mouse brain.

STEM121在小鼠大腦海馬中檢測移植的人類神經(jīng)干細(xì)胞的遷移和分化。

STEM123 detects human GFAP+ astrocytes differentiated from human neural stem cells after transplantation into a mouse brain.

STEM123在小鼠腦中檢測移植的人類神經(jīng)干細(xì)胞分化的人類GFAP +星形細(xì)胞。

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參考文獻(xiàn)

1. Cummings BJ, et al. (2005) Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS 102: 14069-14074.

2. Guzman R, et al. (2007) Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI. PNAS 104: 10211-10216.

3. Tamaki SJ, et al. (2009) Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell 5:310-319.

4. Salazar DL, et al. (2010) Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE 5: e12272.

5. Kelly S, et al. Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. PNAS (2004) 101:11839 11844.

6. Cummings BJ, et al. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS (2005) 102: 14069-14074.

7. Tamaki SJ, et al. Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell (2009) 5:310-319.

8. Kallur T, et al. Human Fetal Cortical and Striatal Neural Stem Cells Generate Region-Specific Neurons In Vitro and Differentiate Extensively to Neurons After Intrastriatal Transplantation in Neonatal Rats. J Neurosci Res. (2006) 84:1630-1644.

9. Salazar DL, et al. Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE (2010) 5: e12272.

10. Tamaki SJ, et al. (2009) Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell 5:310-319.

11. Salazar DL, et al. (2010) Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE 5:e12272.

12. Cummings BJ, et al. (2005) Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS 102: 14069-14074.

背景介紹

Stem121：抗人類細(xì)胞質(zhì)蛋白的小鼠抗體

1. Stem121是什么？

· Stem121是小鼠來源的單克隆抗體，它特異性地識(shí)別人類細(xì)胞（特別是人類神經(jīng)細(xì)胞）細(xì)胞質(zhì)中的某個(gè)抗原。

· Stem121在反應(yīng)屬性上有兩個(gè)主要特點(diǎn)：

    1) 它所識(shí)別的抗原不在細(xì)胞核內(nèi)，可以特異性的識(shí)別細(xì)胞質(zhì)內(nèi)的抗原；

    2）它與小鼠、大鼠、猴子細(xì)胞的細(xì)胞質(zhì)沒有反應(yīng)性。

2. Stem121的應(yīng)用

· 小鼠、大鼠、猴子是目前干細(xì)胞治療研究者常用的模式動(dòng)物。通常，研究者將人類的神經(jīng)干細(xì)胞注射進(jìn)入這些模式動(dòng)物的腦部，然后觀察這些細(xì)胞是否可以在動(dòng)物的腦部存活、生長、分化、遷移。因此，必須可靠而且準(zhǔn)確地示蹤在動(dòng)物體內(nèi)的人類神經(jīng)干細(xì)胞。

· Stem121抗體是可以對人類細(xì)胞細(xì)胞質(zhì)進(jìn)行標(biāo)記的抗體。

· 對于輸入的人神經(jīng)干細(xì)胞，既標(biāo)記細(xì)胞核又標(biāo)記細(xì)胞質(zhì)，意味著更加可靠、更加準(zhǔn)確。如下圖所示，人類的神經(jīng)干細(xì)胞被注射進(jìn)入大鼠的腦部，藍(lán)色標(biāo)記是某種識(shí)別人類細(xì)胞的細(xì)胞核的抗體，綠色標(biāo)記是識(shí)別人類細(xì)胞細(xì)胞質(zhì)的Stem121抗體，紅色標(biāo)記是識(shí)別人類細(xì)胞某種發(fā)炎癥狀的抗體。在A部分的左圖左下角有個(gè)箭頭指示的細(xì)胞，具備藍(lán)色與綠色標(biāo)記，這說明這個(gè)細(xì)胞確定是人類細(xì)胞而非大鼠細(xì)胞；右圖右下角的箭頭指示同一個(gè)細(xì)胞，沒有綠色標(biāo)記，說明這個(gè)人類細(xì)胞并沒有出現(xiàn)發(fā)炎癥狀。而且，如果實(shí)驗(yàn)的設(shè)計(jì)目的是觀察人類的神經(jīng)細(xì)胞的細(xì)胞質(zhì)能否進(jìn)入到人類或是動(dòng)物細(xì)胞的細(xì)胞核里面，那么就需要Stem121對人類細(xì)胞質(zhì)進(jìn)行標(biāo)記。

· 另外，需要注意，Stem121在之前發(fā)表的文章中，也被稱作SC121。（Kelly S, et al .Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. PNAS,2004,101: 11839-11844.）

訂購信息

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

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